پديد آورندگان :
Omrani نويسنده , Samadzadae Saied نويسنده , Farshid نويسنده , Jahandidae نويسنده , Yazdanpanah M.M. نويسنده
چكيده لاتين :
A significant proportion of infertile men with azoospermia and severe oligoazoospermia have a genetic
etiology for their reproductive failure. Genetic analysis has major effects on finding the eauses of infertility in last decade,
but still in some cases , we still do not have clear answer for our patients. During last years it has become evident
that endogenous estrogens and estrogen receptors (ER) play role in the regulation of testicular function. Present study
was performed to evaluate the significance of Rsal and AluI single nucleotide polymorphism in the ERfJgene in infertile
patients in comparison with normal fertile male control.
Methods: From 120 infertile men referred to our center after ruling out all the known causes of the infertility such as
chromosomal abnormalities, V-chromosome microdeletion, and other pathologie disorders, 5 rnl peripheral blood were
obtained for DNA extraction. PCR amplification of the polymorphic region was carried out and after running the PCR
products on 1.5% agarose gel, the frequency of the polymorphism were calculated.
Results: A 3 times higher frequency of the heterozygous Rsal:genotype was found in men with low sperm concentration
compared to control (P=0.003). In contrast, the proportion of homozygous Alul genotype was only 113 in severely
oligoazoospermic men in comparison with control (P=0.03) .
Conclusion: Our results could suggest that ERfJand Rsal and AluI single nucleotide polyrnorphisms on this gene are
important for spermatogenesis in humans, and could play an important role in the spermatogenesis process in males .
Also it is possible to conclude that different conditions of infertility may not have genetic predisposition in common.