عنوان به زبان ديگر :
Derivative Spectrophotometry for Simultaneous Analysis of Chlorpheniramine Maleate, Phenylephrine HCl, and Phenylpropanolamine HCl in Ternary Mixtures and Pharmaceutical Dosage Forms
چكيده لاتين :
In this study, different derivative spectrophotometric methods are proposed for the
simultaneous determination of chlorpheniramine maleate (CP), phenylephrine HCl (PE) and
phenylpropanolamine HCl (PP) in their ternary mixtures and in pharmaceutical dosage forms.
Spectra of single component and ternary mixtures of various concentrations and combinations
from zero- to fourth-derivation were obtained. Also the spectra of the excipients including
lactose, starch, and microcrystalline cellulose were obtained to study the possible interference
from matrices. Zero-crossing derivative spectrophotometry based on recording the secondderivative
curve for PE at 286.5 DID and fourth-derivative curve for PP at 220 nm were used
for determining each component. Third component, CP,was determined by measuring absolute
amplitudes at 265.8, 262.2, 269.5, and 273.8 nm in its second derivative spectra. Results
showed that the matrices have no interferences. The calibration curves were linear in the range
of 1-8 ug/ml for PE; 5-30 ug/ml for PP; and 2-8 ug/ml for CP. The limits of detection were
0.2 ug/ml for PE, 0.1 ug/ml for PP, and 0.3 ug/ml for CP. The mean percentage recoveries
obtained for different synthetic mixtures by using this method were 95.3% with coefficient
of variation of 4.3% for PE, 101.5% with coefficient of variation of 1.4% for PP, and 99.4%
with coefficient of variation of 1.5% for CP. This method has been applied successfully for the
determination of PE and PP in its combination with CP in Antihistamine Decongestant tablets
with a high percentage of recovery, good accuracy and precision.
