مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

The aim of the present study is to develop a new culture system, capable of producing healthy preantral follicles, which can reach full maturity especially in a long-term culture. To achieve this task we used CRI aa medium in combination with endocrine hormone (FSH), growth factor (EGF) and/or Insulin-TransferrinSelenium (ITS). More over, we compared its efficacy with TCM-199 medium plus the same supplements. Culturing of the preantral follicles with diameters of 60, 80 and 100 urnwere commenced at follicle recovery (day 1) and continued for 26 days. Follicle diameters and morphological deformity or degeneration were monitored on the day of collection and during the culture (days 5, 10, 15, 20 and 26). Compared with TCM-199 medium, culture system using CRlaa medium showed a significant increase in the size and viability of the follicles of all size classes regardless of the used supplements. The follicular survival rate at 26th day of the culture in CR1aa culture system reached 28%. Regardless of culture treatments, the greatest follicular growth rate was between days 5, 10 and 15 of culture. The survived follicles maintained their morphology throughout the culture, with presence of a thecal layer and basement membrane surrounding the granulosa cells. In conclusion, CRlaa medium plus FSH, EGF and/or ITS can be a promising culture system for long-term culture of bovine preantral follicles.