چكيده لاتين :
Background: The aim of the present study was to investigate the role of progesterone on the developmental competence of cumulus-oocyte complexes (COCs) and cumulus-denuded oocytes «(יDOs) at germinal vesicle (GV) stage. Materials and Methods: GV oocytes of pregnant mareיs serum gonadotropin (PMSG)-primed prepubertal mice were divided into two groups: CDOs and COCs. The oocytes were cultured
in TCM 199 with different concentrations of progesterone (10, 38, 50 and 100 JlM) and without progesterone (controls). The number of oocytes at the GV, germinal vesicle breakdown (GVBD) and metaphase II (MIl) stages were counted. In vitro fertilization (IVF) of MIl oocytes and their development to the blastocyst stage were evaluated.
Results: Significantly different MIl rates were observed between the COCs (85%) and COOs (68%) control groups. The MIl rates of 83%, 480/0, 14% and 00/0 for COCs and 650/0, 53%, 200/0 and 00/0 for COOs were obtained in TCM 199 that contained 10, 38, 50 and 100JlM progesterone concentrations, respectively. These MIl rates were lower (p<0.05) in both COCs and COOs as compared to their respective control groups, except for 10 JlM. The fertilization and blastocyst rates
ofCOCs (830/0 and 350/0, respectively) were higher (p <0.05) than those ofthe CDOs (51% and 50/0, respectively) control groups. The fertilization and blastocyst rates in the presence of 10 JlM (81%
and 36%, respectively) and 38 JlM (85% and 30%, respectively) progesterone in COCs and COOs (52% and 40/0 for 10 JlM; 56% and 4% for 38 JlM) were similar to their respective control groups.
Conclusion: Adding progesterone to the medium could not improve maturation of mouse GV oocytes and their development to the blastocyst stage.
