مرکز منطقه ای اطلاع رساني علوم و فناوري -

چكيده لاتين :

Six insect cell lines were tested for susceptibility to Syngrapha falcifera multiple nucleocapsid nucleopolyhedrovirus-D (SfaMNPV-D) infection by use of a typical endpoint assay procedure. Cell lines from Trichoplusia ni (TnSBl-4), (Ll 05-clone), Spodoptero lituro (SL-ZSU-1), Spodoptero jrugiperdo (IPLB-SF-21), Pieris ropoeb (Pr-E-HNU9) and Helicoverpo zeo (BCIRL-HZ-AM1) in 96-well tissue culture plates were infected with dilutions of extra cellular virus suspensions of (SfaMNPV-D). Each cell/virus combination was incubated at temperatures 27°C and wells were scored for positive infection at 2 to 4 day intervals. The resulting data were analyzed by Reed and Muench method, providing virus titers for each combination of virus, cell line. The results were categorized by accuracy and by rapidity of maximum titer. Virus titer of TnSB-4 was higher than other cell lines TCID 50 8.7 x10^8 , the lowest level detected in infected was in (Pr-E-HNU9) cells TCID50 2.4x10^8 . No Virions or polyhedral inclusion bodies were detected in infected SL-ZSU-1 cells.