چكيده لاتين :
Background and Objectives: Making stacking gels for polyacrylamide gels in the laboratory
by conventional methods is laborious and time consuming. Considering the role of temperature in
polyacrylamide gels with respect to electrical resistance and viscosity, we assumed that decreasing
the temperature would cause an increase in electrical resistance and viscosity. Ultimately, a
downward temperature gradient imposed in the first phase of polyacrylamide gel running time
would supposedly improve the migration of macromolecules. This project analyzed the effect of
temperature gradient on the migration of macromolecules in the continuous gels (without stacking)
and compared it with results obtained using stacking gels.
Material and Methods: Electrical resistance was calculated using Ohm’s law. Subsequently, to
examine the effect of temperature change on macromolecules separation, conformation sensitive
gel electrophoresis (CSGE) was used as a model and specimens were run under three different
conditions, one of which was prepared with a stacking gel.
Results: The electrical resistance showed an inverse relationship with temperature in this study.
Separation of the DNA molecules in the continuous gels (with no stacking) was comparable with the
conventional method (with stacking).
Conclusion: Using a temperature gradient against time may be an alternative method for
stacking gels.
