Detection of virulence factors of Pseudomonas aeruginosa in different animals by using bacteriological and molecular methods

The aim of this study was to detect the presence of virulence factors of Pseudomonas aeruginosa in different animals. Forthis purpose 120 samples were collected and examined to detect fourteen virulence factors by using biochemical andmolecular methods. The results showed that the highest isolation rate was recorded in doges (29.6%) among studied animals,and highest isolation rate was recorded in milk samples (26.8%) among the studied samples. The virulence factors weredetected in different ratio, and highest of them were capsule detected in 50% from skin isolates, amylase enzyme detected in28.5% from milk isolates, hemolysin enzyme detected in 75% from wound isolates, protease detected in 100% from skinisolates, phospholipase enzyme detected in 56.1% from milk isolates, urease enzyme detected in 50% from skin isolates,gelatin liquefaction detected in 100% from skin and ear isolates, β -lactamase production detected in 100% from skin andwound isolates, pigments production detected in 100% from skin and ear isolates, oprI, oprL and exoT detected in 100% fromskin and wound isolates, exoS detected in 100% and 85.7% from skin and milk isolates respectively. We conclude from hisstudy that the dogs are more sensitive in compare with studied animal, while the milk sample is more susceptible tocontamination by Pseudomonas aeruginosa. Regarding the virulence factors we noticed that the appearance of it basis oninfection state.