Immunodetection of Ostertagia ostertagi in Cattle Milk Samples in Wasit Province

The aim of present study was to detect the seroprevalence of Ostertagia ostertagi specific antibodies in cattle milk samples in Wasit province, by using the indirect ELISA test for first time in Iraq. For this purpose, an overall 368 dairies cow was submitted for study and the results were revealed that 51 (13.86 %) of tested cows were positive, and the mean optic density ratios (ODRs) of ELISA test values in seropositive cattle were 0.58. Also, this study aimed to investigate an association of seropositive results with some epidemiological risk factors. Hence, the positive results, according to these factors, were as follow: in milk production factor, 6.32 % for ≥18 liters/day group, 14.29 % for ≥10-18 liters/day group, and 25 % for ≥ 10 liters/day group; in age factor, 13.41% for ≥3-6 years group, and 14.75% for 6 years group; in breed factor, 18.27% for local breed group, 12.17% for cross-breed group, and 12% for pure breed group; in farm management factor, 21.35% for bad management group, and 5.68% for good management group; and in herd size factor, 11.59% for 25 (cow/herd) group, and 17.78% for ≥ 25 (cow/herd) group.Statistically, the significant differences (P≤ 0.05) were observed among related groups of milk production, breed, husbandry management, and herd size factors; while it’s not reported among groups of age factor.