بررسي مولكولار اپيدميولوژي شيگلا سونه اي جدا شده از موارد باليني در تهران با روش RAPD - PCR

بیماری شیگلوز كه بوسیله انواع سویه های شیگلا ایجاد می شود اغلب در كودكان زیر ۱۵ سال دیده می شود كه اغلب با كاهش رشد كودكان و سندروم اورمی هموليتيك همراه می باشد. در این مطالعه كه در طول سالهای 1388 تا 1392 در بیمارستان های تهران بر روی افراد مبتلا به شیگلوز انجام شد، میزان آلودگی گروه های سنی مختلف به شیگلاسونه ای به روش RAPD-PCR مورد ارزیابی قرار گرفت. در این بررسی جع جمعاً 60 ایزوله شیگلا سونه ای كه مربوط به ۲۶ پسر (60%) و 24 دختر (40%) بودند مورد مطالعه قرار گرفتند. تمامی ایزوله ها به دو روش بیوشیمیایی ( تست حركت، MR، سیترات، H2ST اندول،لیزین دكربوكسیلاز، اورنتین دكربوكسیلاز و ONPG) و سرولوژی مورد تایید قرار گرفت.

Bloody diarrhea (shigellosis) is caused by different species of Shigella and often is seen in children under 15 years old. This disease is very contagious and is epidemic and endemic in communities with low level hygiene, and joined with hemolytic uremia syndrome and decrease of childrenʹs growth. As the rate of infection by shigella sonei among different ranges of age is considered as an indicator of hygiene level, this study was designated to detect the rate of infection by shigella sonei among different ranges of age in Tehran by Random Amplified Polymorphic DNA (RAPD-PCR) between 1381- 1385. In this study totally 60 isolates of shigella sonei isolated from 36 (60%) boys and 24 (40%) girls. All isolates were primary confirmed as shigella species by biochemical (Motility, MR, Citrate, H2S, Indole, Lysin decarboxylase, Ornitin decarboxylase, ONPG) and serologic tests; then all isolates were finally confirmed as shigella sonei by RAPDPCR test. Among all these 60 patients, most rate of infection belonged to 1-2 years old group, so in this group 22 (36.7%) were infected by shigella sonei. Furthermore, the less rate of infection belonged to group more than 9 years old (1.6%). This study showed that, RAPD PCR method has a relative good discrimination power, and according to its high discrimination power, the ability of typing and reproducibility, low costs, rapidity and facility, is a good method for typing of Shigella isolates in molecular epidemiology studies.