Inhibition of Phosphodiesterase I by Natural Flavone, Tricin

Objectives: Nucleotide pyrophosates/phosphodiesterases (NPPs) are hydrolases that act on diesters of phosphoric acid. They catalyze the release of nucleoside-5′-monophosphates from various phosphate bonds and phosphodiester bonds. The NPP family comprises of three members known as NPP-1, NPP-2 and NPP-3. They are widely distributed in mammalian intestinal mucosa, liver cells, and serum, snake venom and in various plants. NPP-1 has been localized on cells of the distal convoluted tubule of kidney, chondrocytes, osteoblasts, epididymic and hepatocytes. NPP1 is a key regulator of calcification of bone and other tissues. Overexpression of NPP1 has been associated with chondrocalcirosis, while under expression causes severe periarticular calcification in mice and the syndrome of idiopathic infantile arterial calcification in human.Materials Methods: 4’, 5, 7-Trihydroxy-3 , 5’-dimethoxyflavone (Tricin), a naturally occurring flavone, was isolated from Alliumatroviolaceum by column chromatography and recrystallization method. The chemical structure of isolate was elucidated on the basis of spectroscopic techniques. We investigated in-vitro inhibitory property of tricin against snake venom Phosphodiesterase I.Also EDTA was used as positive controls.Results Conclusion: Tricinas natural flavone, showed strong inhibitory potential with IC50 of 118.3± 5.5 mM as compared to EDTA (IC50 265± 1.48). NPPs are believed to be convoluted in a wide variety of processes, such as bone formation, insulin resistance and metastasis of cancer cells. Inhibitors of PP-1 might be useful as treatment for some forms of arthritis. Only a few inhibitors of Phosphodiesterase I have been reported so far and the majority of them are synthetic in origin. Tricin is found to be a good inhibitor and plays a significant role in treatment diseases especially Alzheimer, epilepsy and Parkinson’s diseases. ,