Novel sorption-based on magnetic nanocomposite monolithic stir bar kit coupled to high performance liquid chromatography-ultraviolet detection for the determination of organophosphorus pesticides in environmental samples by optimizing the central composite design

In this study, a novel stir bar sorptive kit based on magnetic nanocomposite monolithic material (MNM) was provided.The size and morphology of synthesized kits were characterized using field emission scanning electron microscopy (FESEM) equipped with energy dispersive X- Ray (EDX) spectroscopy system. The extraction and desorption analytical parameters, were optimized using a five-level three factor central composite design (CCD) and systematically, respectively. The prepared MNMSB kit was characterized and good preparation reproducibility was obtained with the relative standard deviations (RSDs) ranging from 3.73% to 7.97% (n = 3) in one batch, and from 5.39% to 10.84% (n = 3) among different batches. At optimum conditions method has linear response over 0.1–30 μg/mL for Ethion and 0.01–12 μg/mL for Phosalone, 0.1–25μg/mL for Diazinon, 0.06–7 μg/mL for Hexythiazox, 0.1–15 μg/mL for Chlorpyrifos and 0.1–10 μg/mL for total of OPPs, respectively. The limits of detection (LODs, S/N = 3) were found to be in the range of 9.60×10-6 μg/mL to 2.97×10-3μg/mL. The RSDs of the method were in the range of 2.73–6.40% (n = 3, c = 1 μg/mL all of OPPs and 0.1μg/mL Phosalone) and the enrichment factors were from 2.23 to 14.52-fold.The results showed that MNMSBS kit could enrich the above-mentioned OPPs effectively. The MNMSBSE–LD/HPLC–UV methodology applied for the determination of OPPs real water samples.