Identification and quantification of ciprofloxacin, enrofloxacineanddifloxacin in milk through excitation emission fluorescence and chemometricsmethods

Though there are many analytical methods available to determine antibiotic residues in milk, most of them are rather complicated[1].In this workamethod for simultaneous determination of ciprofloxacin, enrofloxacine and difloxacin in milk was proposed by fluorescence spectroscopy combined with chemometrics. Antibiotics are becoming available for use in animal husbandry. This, along with the possibility of the development of antibiotic resistant human pathogens, make it an important class of drug for which methodology is required for the monitoring of residues in food[2].Rank annihilation factor analysis (RAFA) is employed to analyze matrices of spectrofluorimetric data in two and three component mixture. Annihilation of the contribution of one chemical component from the original data matrix is basis of RAFA. In order to apply RAFA for the determination of an analyte in an unknown sample, a standard second order data of the analyte with rank one should generally be available[3].The determinations were performed in methanol/water medium (60/40% v/v) at pH 9.1, provided by adding a tris buffer solution in the difference concentration of antibiotic samples. Using RAFA for excitation–emission matrix fluorescence successfully achieved quantitative analysis of the three residues antibiotics simultaneously. This algorithm achieves the second-order advantage, and is in principle able to overcome the problem of the presence of unexpected interferences [4].