ساختار تشريحي و بافت شناسي كليه و آبشش در كوسه ماهي عربي (Chiloscyllium arabicum)

كوسه ماهي عربي از خانواده Hemiscyllidaeو جنس chilioscylliumمي باشد كه داراي بدني بلندوباريك و بسيار مقاوم به بي آبي مي باشد هدف از اين تحقيق مطالعه ساختار تشريحي و بافت شناسي كليه و آبشش كوسه ماهي عربي(c.arabicum)مي باشد كه بدين منظور تعداد15عدد كوسه ماهي عربي از شمال خليج فارس صيد گرديد و به طور زنده به ازمايشگاه علوم وفنون خرمشهرمنتقل شدند كه پس از تشريح و تثبيت در ماده ثبوتي بوئن به مدت 72 ساعت جهت مراحل آبگيري،شفاف سازي وپارافينه كردن درون دستگاه هيستوكينت قرار گرفتند وپس ازمونته كردن و قالب گيري توسط دستگاه ميكروتوم برش داده شدند و پس از رنگ آميزي با هماتوكسيلين وائوزين با ميكروسكوپ نوري مورد مطالعه گرديدو مشاهده شد كه كليه كوسه ماهي عربي همانند ديگر ماهيان استخواني مزونفريك است و داراي سه بخش راس،بدنه و دم مي باشد.آبشش نيز از رشته هاي آبششي كه عمود بر كمان آبششي قرار دارندتشكيل شده است كه هر رشته آبششي لاملاها از دو نوع سلول به نام سلول هاي ستوني يا پيلار و سلول هاي پوششي تشكيل شده اند.

The present invest tigation aimes to study the histomorphological structure of kidney and gill in Arabian bamboo shark , Chiloscyllium arabicum,. In this regard, 30 C.arabicum were caught at the north of Persica Gulf. and moved to laboratory alive. The fish were discected and samples were taken from the gill and kidney and fixed in the Buine,s Solutions for 72h. Then, tissuve samples concentration of ethanol, cleared in xylon and finaly embded in parafine using tissue processor operators RX11B There after 5-6 Ҷm tissue sections were prepared using rotary microtom( Leica RM2245 . and stained with hematoxylin and eosin (H E). The stuined sections were finaly evaluated using digital light microscopy with Dino capture saftweres and photo micrographs were obtained. The result showed that the kidney was mesonephric in C.arabicum same as teleost. The kidney consisted of head, trunk and tail. Nephrones with renal corpuscles and renal tubuls were observed in the kidney trunk. The gill consisted of filaments which were covered with the gill lamellae. Lamellae were couted with the pavement cells and pillar cells were horizontally placed in the lamellae. The choloricd cells were placed in the basic of the filaments. In conclusion, the basic structure of gill and kidney in C.arabicum was similar to that in teleosts.