Voltammetric determination of trace amount of norepinephrine using modified carbon paste electrode

The electrochemical determination of norepinephrine (NE) as a biomolecule has been investigated in the current work. NE is one of the derivatives of cathecholamines secreted in the adrenal medulla and plays important physiological roles in the central nervous. Hence, it is very necessary to develop sensitive, selective and practically reliable method for the direct determination of the NE dosage for monitoring physiological activities and diagnosing diseases [1-3]. The current work deals with fabrication of a high sensitive modified carbon paste electrode employing CdO nanoparticles and 1-butyl-3-methylimidazolium tetrafluoroborate (1B3MITFB) (CdO/NPs/1B3MITFB/MCPE) for determination of trace amount of NE using cyclic voltammetry (CV) and square wave voltammetry (SWV) techniques. After optimization of electrochemical analytical conditions using a CdO /NPs/1B3MITFB/MCPE at pH 7.0 phosphate buffer (0.1 M), the oxidation peak current was found to vary linearly with its concentration in the range of 20.0-300 μM for NE and satisfactory lower detection limit of 8.0 μM NE. The CdO/NPs/1B3MITFB/MCPE showed several advantages such as good reproducibility, low limit of detection (LOD) and simple preparation. The novel proposed sensor was employed for the analysis of NE in real samples. The synthesized CdO nanoparticles were characterized with different methods such as SEM, XRD and EDAX.