Determination of Ceftizoxime Using Reduced Graphene Oxide Hollow Gold Nanosphers (RGO-HGNs) Electrode by Differential Pulse Voltammetry

At first Graphene Oxide (GO) was reduced on the surface of electrode by electrochemical method then reduced graphene oxide-hollow gold Nanosphers (RGO-HGNs) were generated onto pencil graphite electrode (PGE). HGNS was produced by electrodeposition and subsequent displacement reaction between the cobalt nanoparticle and 𝐴𝑢3+ions (1). Differential pulse voltammetry was used for determination of ceftizoxime (CFX). CFX is a third-generation cephalosporin available for parenteral administration CFX that is used to reduce the infection caused by both gram-negative and gram positive bacteria, by interfering with the formation of the bacterial cell wall so that the wall ruptures, resulting in the death of bacteria. The design experiment was a central composite design (CCD) methodology was developed as experimental strategies for modeling and optimization of the influence of some variables on the performance of modified electrode (2). The dynamic range was 1nM to 100 nM. The present method has good selectivity and sensitivity. It has been applied to the determination of CFX in real sample.