ﺗﻌﯿﯿﻦ اﻟﮕﻮي ﻣﻘﺎوﻣﺖ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮑﯽ در ﺟﺪاﯾﻪﻫﺎي آﺋﺮوﻣﻮﻧﺎس ﻫﯿﺪروﻓﯿﻼ در ﮐﭙﻮر ﻣﻌﻤﻮﻟﯽ (Cyprinus carpio)

Determination of antimicrobial resistance profile in Aeromonas hydrophila isolates in common carp (Cyprinus carpio)

در آﺑﺰيﭘﺮوري، ﺑﺎﮐﺘﺮيﻫﺎي ﻓﺮﺻﺖﻃﻠﺐ در ﻫﻨﮕﺎﻣﯽ ﮐﻪ ﺑﻪ ﻋﻠﺖ اﺳﺘﺮس ﯾﺎ ﻋﻮاﻣﻞ ﺑﯿﻤﺎري زاي دﯾﮕﺮ، ﻣﺎﻫﯽ ﻣﺴﺘﻌﺪ ﺑﻪ اﺑﺘﻼ ﺑﻪ ﺑﯿﻤﺎري اﺳﺖ، اﯾﺠﺎد ﺑﯿﻤﺎري ﻣﯽﮐﻨﻨﺪ. ﺷﺎﺧﺺﺗﺮﯾﻦ ﮔﺮوه از اﯾﻦ ﻣﯿﮑﺮوارﮔﺎﻧﯿ ﺴﻢﻫﺎي ﻓﺮ ﺻﺖ ﻃﻠﺐ، دﺳﺘﻪي آﺋﺮوﻣﻮﻧﺎسﻫﺎي ﻣﺘﺤﺮك ﻣﯽﺑﺎﺷـــﺪ ﻣﺤﺪود ﮐﺮدن ﺗﺠﻮﯾﺰ آﻧﺘﯽﺑﯿﻮﺗﯿﮏ ﻣﻬﻢﺗﺮﯾﻦ ﻋﺎﻣﻞ ﺑﺮاي ﭘﯿﺶﮔﯿﺮي از ﻣﻘﺎوﻣﺖ داروﯾﯽ اﺳـــﺖ، ﺗﻌﯿﯿﻦ اﻟﮕﻮي ﻣﻘﺎوﻣﺖ آﻧﺘﯽﺑﯿﻮﺗﯿﮑﯽ در ﺑﺎﮐﺘﺮيﻫﺎي ﺑﯿﻤﺎري زاي ﺷﺎﯾﻊ ﺟﻬﺖ ﻫﺪاﯾﺖ درﻣﺎن ﺗﺠﺮﺑﯽ و اﺧﺘﺼﺎ ﺻﯽ ﻋﻠﯿﻪ ﯾﮏ ﭘﺎﺗﻮژن ﺧﺎص ﺣﺎﺋﺰ اﻫﻤﯿﺖ اﺳﺖ. ﺳــﻮﯾﻪ ﻫﺎي ﻣﺨﺘﻠﻒ آﺋﺮوﻣﻮﻧﺎس ﻫﯿﺪروﻓﯿﻼ در ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿــﺮ از ﮐﭙﻮر ﻣﺎﻫﯿﺎن ﻣﺰارع ﭘﺮورش ﻣﺎﻫﯽ اﺳــﺘﺎن ﺧﻮزﺳــﺘﺎن ﮐﻪ داراي ﻋﻼﺋﻢ ﺑﺎﻟﯿﻨﯽ و ﺳــﭙﺘﯽ ﺳــﻤﯽ ﺑﻮدﻧﺪ، ﺟﺪاﺳــﺎزي ﺷــﺪﻧﺪ ﺟﻬﺖ ﺗﻌﯿﯿﻦ ﺟﻨﺲ آﺋﺮوﻣﻮﻧﺎس از ﭘﺮاﯾﻤﺮﻫﺎي اﺧﺘﺼــﺎﺻــﯽ ژن و ﺟﻬﺖ ﺗﺎﯾﯿﺪ ﮔﻮﻧﻪ ﻫﯿﺪروﻓﯿﻼ از ﭘﺮاﯾﻤﺮﻫﺎي اﺧﺘﺼﺎﺻﯽ ژن srRNA16 ﻫﯿﺪروﻓﯿﻼ و ژن ﻟﯿﭙﺎز اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. ﺟﻬﺖ ﺑﺮرﺳﯽ ﻣﻘﺎوﻣﺖ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮑﯽ ﺳﻮﯾﻪ ﻫﺎي ﺗﺎﯾﯿﺪ ﺷﺪه از روش اﻧﺘﺸﺎر دﯾﺴﮏ ﮐﺮﺑﯽ-ﺑﺎور اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. ﺑﺎ ﺑﺮرﺳﯽ ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از آﻧﺘﯽ ﺑﯿﻮﮔﺮام ﻣﺸﺎﻫﺪه ﺷﺪ ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻣﻘﺎوﻣﺖ در ﺑﯿﻦ ﺟﺪاﯾﻪﻫﺎي ﻣﻮرد ﺑﺮرﺳـــﯽ ﻣﺮﺑﻮط ﺑﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏﻫﺎي ﮐﻠﯿﻨﺪاﻣﺎﯾﺴـــﯿﻦ (90 %) ، ، آﻣﻮﮐﺴـــﯽ ﺳـــﯿﻠﯿﻦ ،(% 37/5) ، اﺳﺘﺮﭘﺘﻮﻣﺎﯾﺴﯿﻦ (10 %)، ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ (5 %)و ﮐﻤﺘﺮﯾﻦ ﻣﻘﺎوﻣﺖ در ﺑﯿﻦ ﺟﺪاﯾﻪﻫﺎ در ارﺗﺒﺎط ﺑﺎ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏﻫﺎي ﺳﯿﭙﺮوﻓﻠﻮﮐﺴﺎ ﺳﯿﻦ 0%(، ﺳﻮﻟﺘﺮﯾﻢ (0%)، اﮐﺴﯽ ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ (0%) ﺑﻮد. ﻫﻤﭽﻨﯿﻦ ﻣﻘﺎوﻣﺖ ﻧﺴﺒﯽ ﻧﺴﺒﺖ ﺑﻪ آﻧﺘﯽ ﺑﯿﻮﺗﯿﮏﻫﺎي ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ، ﮐﻠﯿﻨﺪاﻣﺎﯾﺴﯿﻦ، اﮐﺴﯽ ﺗﺘﺮاﺳﺎﯾﮑﻠﯿﻦ و اﺳﺘﺮﭘﺘﻮﻣﺎﯾﺴﯿﻦ ﻣﺸﺎﻫﺪه ﮔﺮدﯾﺪ.

In aquaculture, opportunistic bacteria cause illness due to stress or other disease factors, when fish are susceptible to the disease. The most prominent group of these opportunistic microorganisms is the category of mobile Aeromonas. The limitation of antibiotic prescribing is the most important factor in preventing drug resistance, determining antibiotic resistance profile in Common pathogenic bacteria are important for conducting experimental and specific treatment against a specific pathogen. Different strains of Aeromonas hydrophila were isolated from cyprinids with clinical signs and septicemia in fish farms of Khuzestan province. To determine the genus Aeromonas, specific primers of the gene were used and to confirm the hydrophilic species from the specific primers of the gene 16 srRNA, hydrophila genus and lipase genes were used. In order to investigate antibiotic resistance of confirmed strains, the Kirby-Disc method was used. By examining the results of antibiogram, the most resistance among the isolates were related to clindamycin (90%), amoxicillin (37.5%), streptomycin (10%), tetracycline (5%) and the least resistance among the isolates were related to ciprofloxacin (0%), sultrim (0%), oxytetracycline (0%). There was also a relative resistance to tetracycline, clindamycin, oxytetracycline and streptomycin antibiotics