Recovery and Enrichment of Alpha Amylase by Batch Foam Fractionation

In this work, the batch foam fractionation of alpha amylase was studied as an alternative to other common applied methods such as chromatography, ultrafiltration, and precipitation techniques and the effects of gas flow rate, surfactant concentration, feed volume and ionic strength on enrichment ratio, recovery and volume ratio were investigated. The parametric studies show that the optimal conditions at steady-sate for the sysytem under investigation were as follows: gas flow rate = 0.3L/min, surfactant concentration = 100 mg/L, feed volume = 580 ml, and ionic strength = 0.05 mol/L.