شماره ركورد كنفرانس :
4518
عنوان مقاله :
Effect of factors on forward extraction of endopolygalacturonase using reverse micelles
Author/Authors :
Reza Rezvaninejad Biotechnology Research Laboratory - School of Chemical Engineering - Iran University of Science and Technology, Tehran , Parisa Hejazi Biotechnology Research Laboratory - School of Chemical Engineering - Iran University of Science and Technology, Tehran
كليدواژه :
Reverse micellar extraction , Endopectinase , One factor at a time method , CTAB
عنوان كنفرانس :
The 7th International Chemical Engineering Congress & Exhibition (IChEC 2011
چكيده لاتين :
Reverse Micellar Extraction (RME) of endopolygalacturonase (endopectinase) from Aspergillus niger using cationic surfactant cetyltrimethylammonium bromide (CTAB) was investigated. The influence of various process factors including aqueous pH, salt and surfactant concentrations on the forward extraction efficiency was studied using one factor at a time method (OFAT). Optimum forward extraction conditions for maximizing efficiencies were in enzyme concentration 500 ppm, pH 8; NaCl concentration 0.10mol.dm−3 and CTAB concentration 150 mmol.dm−3. Under these conditions, forward and backward extraction efficiencies of 71% and 57% were obtained, respectively.
