Naked-eye detection and determination of lysine in representative protein foodstuffs by using murexide-zinc chemosensor based on colorimetric method

Lysine is an essential amino acid, meaning that the human body cannot synthesize it. So we must put human diet. Lysine deficiency, protein synthesis limit. Complications can be caused by lysine deficiency , anemia , enzyme problems , hair loss , inability focusing , irritability , lack of energy , loss of appetite , fertility problems , delayed growth , and weight loss named [1]. Colorimetric sensing which invariably bank on “naked-eye “sensing, has gained additional impetus owing to the ease in monitoring of the process without resorting to sophisticated techniques. Thus a great deal of effort has been invested in developing simple-to-make and use “naked-eye “diagnostic tools for sensing of analytes [2, 3]. These receptors are rather difficult to synthesize or require expensive instruments for detections. Taking this dilemma into consideration recently, we have undertaken an extensive research program to explore bromo pyrogallol red as an easily available dye demonstrated a high chromogenic receptor for cations and anions [4, 5]. In this work, we disclose a new approach toward this goal. n this work, the behavior of murexide (MX), an easily available dye has been studied for the first time chromogenic sensing of lysine as an amino acid species and for Zn2+ as a cationic species in H2O media. So employing MX an efficient colorimetric chemosensor was afforded with a chromogenic selectivity for Zn2+ over other cations with detection limit 0.1 μmol.L-1 lysine among Glycine, Alanine, Serine, Threonine, Valine, Lucien, Isoleucine, Phenylalanine, Tyrosine, Tryptophan, cysteine, Methionine, Glutamic acid, Aspartic acid, Arginine, Histidine, Proline, Glutamine through a fast response time of less than 30 s which is much lower than most recently reported chromogenic probes. The linear dynamic ranges for the determination of lysine were 0.95-46.14 μmol.L-1. This method could have potential application in a variety of cases requiring rapid and accurate analysis of lysine for cheeses samples.