Application of microfluidic technology in cell-free DNA analysis

Circulating free DNA (cfDNA) is the small fragments of a cellular nucleic acids that are detectable in body fluids and are involved in numerous physiological and pathological phenomenon also it can carry signatures (i.e.., mutations) related to certain pathological conditions. Currently, several chip-based electrochemical sensors have been developed for the analysis of ctDNA and cfDNA in patient samples. Microfluidic devices allow for some unique characteristics compared to benchtop approaches making them attractive platforms for clinical use, such as low processing time, closed architecture to minimize sample contamination, conducive to automation and the ability to allow for process integration including downstream molecular analysis. Method: In this review we gathered data from Scopus, Science Direct, Embase and NCBI databases about 50 paper was collected by applying time filtration eventually 10 paper was reviewed which was near for our purpose. Result: Here we describe the importance of cfDNA analysis as a potential biomarker of diseases like cancer and microfluidic developments in cell-free DNA analysis. We also present an overview of current advances in analytical sensitivity and accuracy of cfDNA analysis methods. Conclusion: However, cfDNA and ctDNA analysis remains challenging due to its low concentration. Microfluidic technologies have been playing an important role in the development of circulating cell-free DNA analysis because their sensitivity allows the titration of biomarkers in complex matrices that were thought to be inappropriate for nucleic acids processing.