Preparation of pencil modified with Bismuth-Antimony alloys and application of them in determination of Arginine

Arginine belongs to amino acids, which has a carboxyl group, an amine group and a side chain attached to a similar carbon atom. Arginine plays an important role in depression and also plays a role in nerve activity and cardioprotection. It is used to treat cancer. The synthesis of arginine occurs in humans from the intestinal and kidney axis [1-5]. In this research, the pencil electrode is activated by the application of potentiostatic or potentiodynamic methods the exprimental results showed that pencil electrode generates a broad oxidation peak, while the activated pencil generates shows a sharp peak. In this work, we have tried to advise a precise and low-cost method to detect and measure the amounts of this compound in pharmaceutical samples. The electrochemical oxidation of arginine on the pencil modified with Bismuth-Antimony alloys electrodes using chronoamprometry method at the potential of -0.7 V vs. Ag /Ag Cl reference electrode for 120 seconds in an alloy solution of bismuth and antimony with the same concentration was studied. The metal ratio in alloy was 1:1 and the supporting electrolytnest was a 1 molar sulfuric acid solution. The differential pulse voltammetric method was used to in the potential range of -0.4 to 0.2 V at a scan rate of 20 mV s-1. According to the calibration curve, the linear range was obtained with a differential pulse voltammetric method of 1000 to 9000 µM and a detection limit of 1179 µM for modified electrode. Finally, this electrode was used to measure arginine amino acid in the sample The real spirulina pill was used. A relative standard deviation is obtained 5.3% for the real sample.