Determination of Carbamazepine in Urine and Water Samples using ‎Functionalized Metal-Organic Framework as Sorbent

A stable and porous amino-functionalized zirconium-based metal organic framework (UiO-66-‎NH2) containing Missing Linker Defects was prepared, and fully characterized by FTIR, scanning ‎electron microscopy (SEM), powder X-ray diffraction (PXRD), and N2 adsorption/desorption ‎isotherm. The Zr-MOF was then applied as an adsorbent in pipette-tip solid phase extraction (PT-‎SPE) of carbamazepine. Important parameters affecting extraction efficiency such as pH, sample ‎volume, type and volume of eluent, amount of adsorbent, and number of aspirating/dispensing ‎cycles for sample solution and eluent solvent were investigated and optimized. The best extraction ‎efficiency was obtained with 5 mg of sorbent at pH 7.5, and 10 µL of eluent solvent when 100 µL ‎of sample solution was used. Linear Dynamic Range (LDR) was obtained between 0.1- 50 µg. L -1 ‎and Limit of Detection (LOD) for 10 measurement of blank solution was 0.05 µg. L -1. This ‎method was successfully applied for extraction of carbamazepine in urine and water samples. The ‎strategy combined the advantages of both PT-SPE and Zr-MOF-NH2 material such as fast and easy ‎operation, low cost, less solvent consumption, large porosity, and large specific surface area.‎