شماره ركورد كنفرانس :
3550
عنوان مقاله :
Glycine capped gold nanoparticles for detection of Pseudomonas aeruginosa by ELISA and UV-Vis spectrophotometry
پديدآورندگان :
Pourreza Nahid npourreza@scu.ac.ir Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran, , Abasipanah Parichehr Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
كليدواژه :
Pseudomonas aeruginosa , Alginate , Glycine , AuNPs , Spectrophotometry , ELISA
عنوان كنفرانس :
بيست و پنجمين سمينار ملي شيمي تجزيه انجمن شيمي ايران
چكيده فارسي :
Pseudomonas aeruginosa is a common bacterial, opportunistic and gram negative bacterium that infects people with poor immune systems and often causes hospital infections and pandemic and general infections. Pseudomonas aeruginosa is currently the most common and deadliest cause of death among various factors in hospital infections. Pseudomonas has a higher relationship with Staphylococcus aureus and other gram-negative bacteria than with deaths from blood-borne infections. Even a small number of these rod bacteria (about 10-100) can form colonies in the colon and cause patients with poor immune systems to develop severe illnesses. In the food experiments, the World Health Organization (WHO) has identified the concentration of Pseudomonas aeruginosa as an indicator of water contamination and water quality determinations. Therefore, the early diagnosis of Pseudomonas aeruginosa is very important [1, 2]. In this work, we have designed a novel method for the synthesis of alginate-AuNPs functionalized by glycine, in order to detect and measure pseudomonas aeruginosa. The AuNPs were synthesis with slowly and dropwise adding of proper amounts of borax solution into the mixture of alginate-Aucl4- as the base matrix. The characteristics of the synthesized alginate-AuNPs was studied by TEM, particle size analyser and UV-Vis spectrophotometry. The effect of various parameters on the measurement of Pseudomonas aeruginosa such as the capping time of gold nanoparticles by glycine, Glycine consumption time and concentration was studied and optimized. The absorption spectra showed that the maximum wavelength of capped nanoparticles was 535 nm. Under the established optimum conditions, the calibration curve was linear in the range of 0.1-3.3 McFarland (r=0.9981) with a detection limit of 0.0976 McFarland. The relative standard deviation for 0.9 and 1.5 McFarland of Pseudomonas aeruginosa was 4.71 and 2.11 % (7 replicate measurements). The sensing system was sucessfully applied for the determination of Pseudomonas aeruginosa in various samples. This measurement process was fast, easy and without using any toxic or harmful materials and solvent, relative to the other usual methods of Pseudomonas aeruginosa measurement.