Embryo germination and proliferation in vitro of Juglans regia L

This paper presents the optimal culture conditions for the in vitro embryo germination and proliferation of Juglans regia L. Peralta is characterised by its resistance to salinity, lime and by its vigour. The first experiment determined the best culture medium for in vitro embryo germination. The Murashige and Skoog medium (MS; Murashige, T., Skoog, A revised medium for rapid growth and bioassays with tobacco tissues cultures. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by the use of shoot tip culture. The best germination percentage was obtained in WPM (81%) with significant differences between the different media. In the second experiment, the optimal benzylaminopurine and indole butyric acid concentrations were determined for the proliferation stage of the explants obtained in the first experiment. The proliferation rates obtained varied from 0 in the medium without cytokinins to 6 in the medium with 0.5 mg l_1 BAP. The cluster proliferation quality and other parameters studied indicate that the optimal treatment was 0.5 mg l_1 BAP.