شماره ركورد كنفرانس :
5041
عنوان مقاله :
Influence of different carbone sources on growth rate of urease producer Staphylococcus sp. IR-103
Author/Authors :
S. Khalaj Department of Industrial Biotechnology - National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran , F. Tabandeh Department of Industrial Biotechnology - National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran , A. Kazemi Department of Industrial Biotechnology - National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran , H. R. Naseri Department of Agriculture and Natural Resources - International Desert Research Center, Tehran University, Tehran, Iran
كليدواژه :
MICP , urease , carbon source , calcium carbonate precipitation
عنوان كنفرانس :
The 10th International Chemical Engineering Congress & Exhibition (IChEC 2018)
چكيده فارسي :
فاقد چكيده فارسي
چكيده لاتين :
The microbial induced calcite precipitation (MICP) has attracted much attention as an environmentally friendly process for soil stabilization. In this process, ureolytic bacteria produce urease enzyme to hydrolyze urea into ammonium and carbonate. The produced carbonate ions are precipitated as calcite crystals in presence of calcium ions and formed connective bridges between sand particles. In previous study, we isolated an indigenous urease producer bacterium named Staphylococcus sp. IR-103 and confirmed its good potential for using in MICP precess. The main target of this study was to design a cost-effective culture medium for growth and urease production of this strain. The bacterial growth as well as the urease activity in a modified M9 minimal medium in presence of different carbon sources, mono- and di-saccharides, were investigated. Then, the stabilization of a poorly-graded (SP) soil in perforated plates treated by bacteria grown on a medium containning different food-grade sugars were meaured. Although the maximum growth and urease activity were obtained in presence of galactose as sole carbon source, but maximum soil strength was achieved while bacteria grown in modified M9+ glucose.
