شماره ركورد كنفرانس :
5041
عنوان مقاله :
In vitro chondrogenic differentiation of human mesenchymal stem cells encapsulated in in situ forming tragacanth hydrogel
Author/Authors :
M. Tavakol Central Iran Research Complex - Nuclear Science and Technology Research Institute, Yazd, Iran , E. Vasheghani-Farahani Biomedical Engineering Division - Faculty of Chemical Engineering - Tarbiat Modares University, Tehran, Iran , M. Soleimanic Departments of Hematology - Faculty of Medical Sciences - Tarbiat Modares University, Tehran, Iran , M. Dehghan-Niri Departments of Hematology - Faculty of Medical Sciences - Tarbiat Modares University, Tehran, Iran
كليدواژه :
Tragacanth , Horseradis proxidase , In situ forming hydrogel , human mesenchymal stem cells , In vitro Chondrogenesis , Cartilage tissue engineering
عنوان كنفرانس :
The 10th International Chemical Engineering Congress & Exhibition (IChEC 2018)
چكيده فارسي :
فاقد چكيده فارسي
چكيده لاتين :
In the present study, an enzyme catalyzed in situ forming hydrogel based on tyramine conjugated tragacanth (TA-GT) was prepared and then utilized, as 3D scaffold, for in vitro chondrotic differentiation of human mesenchymal stem cells (hMSCs). The hydrogel was prepared by mixing of TA-GT, horseradish peroxidase (HRP) and hydrogen peroxide solution, using a double syringe equipped with a mixing chamber. Then, in vitro chondrogenic differentiation of the encapsulated hMSCs, after 21 days incubation in the presence of differentiation medium, were investigated. Hydrogel formation was obtained due to enzyme catalyzed oxidative coupling reaction between phenolic groups of tyramine groups. Viability of the encapsulated hMSCs was more than 90% and 75% after 2 h and 21 days of incubation. The expression of chondrocytic genes and sulphated glycosaminoglycan indicated that the encapsulated hMSCs differentiated to chondrocyte cells during in vitro differentiation time.
