A New Optical Sensor Array Platform Based on Nanozyme: Classification and Determination of Purine Bases

In the last few decades, nanozymes (synthetic enzymes) have attracted the attention of researchers. Compared to natural enzymes, nanozymes have more advantages such as easy production, high stability, cost-effectiveness. Due to their unique properties, peroxidase-mimicking nanozymes can be considered a potential substitute for peroxidase enzymes in the measurement and analysis of biological compounds. In this research, we proposed an optical tongue colorimetric sensor array based on 8 pseudo-peroxidation nanozymes for quantitative Classification and measurement of purine bases [1]. After the interaction between the nanozymes and analyte and substrate, absorbance at 405 nm wavelength was read by the microplate reader. Then the obtained data were analyzed using chemometrics statistical methods to quantitatively measure and determinate the target purines. In this study, the detection limit for uric acid and adenine analytes was 2.52 μM and 3.63 μM, respectively. The designed nanozyme colorimetric sensor array was able to distinguish purine bases (adenine, guanine, caffeine, xanthine, uric acid, guanosine) well with 100% accuracy using PCA, LDA, HCA pattern recognition chemometrics statistical methods. The diagnostic system based on peroxidation nanograms can be a suitable candidate for the development of multifunctional sensors.