پديدآورندگان :
Gholizadeh-Vazvani Mozhgan mozhgan.gholizadeh@stu.vru.ac.ir Vali –e- Asr University of Rafsanjan, Iran, Rafsanjan , Saberi Riseh Roohallah r.saberi@vru.ac.ir Vali –e- Asr University of Rafsanjan, Iran, Rafsanjan , Dashti Hossein dashti@vru.ac.ir Vali-e- Asr University of Rafsanjan, Iran, Rafsanjan
كليدواژه :
glutenin subunit , correlation , 1000 , seed weight , similarity coefficients
چكيده فارسي :
Variation of high molecular weight glutenin subunits (HMW-GS) at the Glu-A1, Glu-B1, and Glu-D1 loci was studied using SDS-PAGE electrophorese in 17 genotypes and cultivars (Pishgam, Chinese spring, Darab, Kavir, Parsi, Mihan, VRU-1382, VRU-408, VRU-468, VRU-1626, VRU-766, VRU-765, VRU-60, VRU-912, VRU-1464, VRU-1801, and VRU-2068. This study aimed to investigate the relationship between glutenin subunits and 1000-seed weight. A good result has obtained the correlation between 1000-seed weight and the null, 5+10, 2+12, and 7+8 alleles. A positive correlation was found between 1000-seed weight and 5+10 allele (r = 0.600), indicating that presence of this allele will increase 1000-seed weight. A negative correlation was found between 1000-seed weight and null, 2+12, and 7+8 alleles (-0.638, -0.595, and -0.508, respectively). Genotypes with lower 1000-seed weight have null alleles, 1, 7+8, and 2+12. Genotypes with higher 1000-seed weight have 2*, 1, 13+16, and 5+10 alleles. Genotypes and cultivars with the least or the most distance can be used as candidate genotypes for heterosis in breeding programs. Pishgam and Parsi cultivars and VRU-1382, VEU-912, VRU-60 and VRU-765 genotypes with similarity coefficients of 0.636 and high 1000-seed weight and 2*, 5 + 10 and 1 glutenin subunits can be used in cross-breeding and hybridization programs to produce valuable heterosis or heterobiosis genotypes.
