بررسي اثر عصاره آويشن كوهي بر مهار نانوفيبريلهاي آميلوئيدي، جهت درمان بيماري آلزايمر

Investigating the effect of Thymus kotschyanus Boiss extract on the inhibition of amyloid nanofibrils for the treatment of Alzheimer s disease

هدف مطالعه حاضر توليد نانوذرات نقره از عصاره آويشن كوهي Thymus kotschyanus Boiss، بررسي اثرات آنتي اكسيداني و مهاري آن بر توليد نانوفيبريلهاي آميلوئيدي بود. ويژگيهاي نانوذرات با روشهاي FTIR،EDX ، DLS، SEM بررسي شدند. اثرات آنتي اكسيداني و تركيبات عصاره با DPPH وGC-MS و ميزان توليد نانوفيبريلها با روش كنگورد بررسي شدند. اندازه نانوذرات nm93-70 بود. بيشترين تركيبات عصاره 4، 5 آلفا–اپوكسي–3– متوكسي (53%/43)، 2-اتيل–پي پريدين–1–متيل (02%/31) و تيمول (65%/23) بودند. غلظتهايmg/ml 8/0 و 1 از عصاره، بيش از %50 DPPH را مهار كرد. بيشترين فعاليت آنتي اكسيداني در نمونه 5، %82/56 و بيشترين مهار توليد نانوفيبريلهاي آميلوئيدي در غلظت mg/ml2، %95 بود.

The aim of the present study was to produce silver nanoparticles from Thymus kotschyanus Boiss extract, to investigate its antioxidant and inhibitory effects on the production of amyloid nanofibrils. Silver nanoparticles were prepared from thyme extract. The characteristics of nanoparticles were investigated by FTIR, EDX, DLS, SEM methods. The antioxidant effects and compounds of the extract were evaluated by DPPH and GC-MS and the production rate of nanofibrils was evaluated by the Concorde method. The size of nanoparticles was 70-93 nm and their maximum absorption was 460 nm. The most compounds of the extract were 5,4-alpha-epoxy-3-methoxy (34.05), thymol (23.65), ethyl-piperidine (20.22). Concentrations of mg/ml (0.8), (1) extract inhibited more than 50% of DPPH. The highest antioxidant activity in sample 5 was 56.82% and the highest inhibition of amyloid nanofibrils production in mg/ml2 concentration was 95%.