پديدآورندگان :
Rahmani Hamidreza Department of Biochemistry, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran , Ghanbariasad Ali Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran , Meshkibaf Mohammad Hassan Department of Biochemistry, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran , Osanloo Mahmoud Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran. m.osanloo@fums.ac.ir
كليدواژه :
A , 375 cells , Skin Cancer , alpha , pinene , Nanotechnology , Electrospinning process
چكيده فارسي :
Aim and Background: Melanoma is the most severe type of skin cancer. It is very important to develop new drugs with few side effects. Alpha-pinene is the main ingredient of rosemary and conifer essential oils. The anticancer activities of α-pinene involve molecular mechanisms such as suppression of inflammation and induction of apoptosis. Methods: Chitosan nanoparticles containing alpha-pinene was first prepared using ionic gelation process. Anticancer effect and effects on apoptotic involved genes were investigated using MTT and RT-qPCR analyses. Results and discussion: Nanoparticles with particle size of 102 ± 6 nm and zeta potential of 41.7 ± 1 millivolts were prepared. ATR-FTIR analysis confirmed the successful loading of α-pinene into the nanoparticles. Free chitosan nanoparticles did not show significant cytotoxic effect ( 10%) on A-375 human melanoma cells. In addition, the effectiveness of chitosan nanoparticles containing alpha-pinene with IC50 of 76.4 µg/ml was significantly more potent than alpha-pinene of the undetermined form with IC50 of 173.5 µg/ml. Moreover, RT-qPCR results showed that Bax/BCL2 ratio after treatment with samples was 1 and apoptosis may have occurred. Conclusion: According to the obtained results, chitosan nanoparticles containing alpha-pinene can be considered for in vivo study.
