ساخت و بررسي نانونيوزوم هاي حاوي عصاره گياه درمنه خزري و ارزيابي سميت آن بر رده سلولي )-7MCF )سرطان پستان

Fabrication and evaluation of Nanoniosomes containing Artemisia annua plant extract and evaluation of its toxicity on breast cancer cell line (MCF-7)

فرموالسيون دارويي نانوذرات به دليل دستيابي به داروي رهاسازي هدفمند و پايدار ، بهبود حالليت دارو و كاهش واكنشهاي جانبي دارويي ، كاربرد زيادي دارد. يكي از راه هاي بهبود بقاي بيماران سرطاني ، درمان با استفاده از نانو حامل هايي مانند نيوزوم براي داروهاي ضد سرطان است. هدف از اين مطالعه، سنتز نانوحامل نيوزوم حاوي عصاره گياه درمنه خزري )annua Artemisia )و بررسي اثرات ضدسرطاني آن است. براي تهيه نيوزوم هاي حاوي عصاره درمنه از روش آب پوشاني اليه نازك استفاده شد سپس خصوصيات فيزيكي و شيميايي آن با استفاده از ميكروسكوپ الكتروني SEM و پراكندگي نور ديناميكي )DLS )مورد بررسي قرار گرفت. هم چنين درصد محصورسازي و الگوي رهايش عصاره با استفاده از كيسه دياليز ارزيابي شد. در انتها اثرات ضد سرطاني عليه رده سلولي سرطان پستان (7_MCF (مورد مطالعه قرار گرفت. نتايج نشان داد نانوحامل سنتز شده حاوي عصاره، داراي ساختار كروي بوده و اندازه آن 104/1 نانومتر و درصد محصورسازي /35 62 % است و داراي اثرات ضدّسرطاني معناداري نسبت به عصاره آزاد ميباشد. نانوداروهايي مانند نيوزوم سيستم هاي انتقال دارو هستند كه براي بهبود اثرات ضد سرطاني و به ويژه غلبه بر سميت شيمي درماني سيستميك ايجاد شده اند و به عنوان سيستم دارورساني هدفمند جهت اهداف ضدسرطاني مورد استفاده قرار مي گيرند.

Background and purpose of the study: pharmaceutical formulation of nanoparticles is widely used due to the achievement of targeted and stable drug release, improvement of drug solubility and reduction of drug side reactions. One of the ways to improve the survival of cancer patients is treatment using nanocarriers such as Niosomes for anticancer drugs. The aim of this study is the synthesis of Niosome nanocarrier containing Artemisia annua plant extract and its anticancer effects. Study method: To prepare the Niosomes containing the extract of fennel, the thin layer water coating method was used, then its physical and chemical properties were investigated using SEM and dynamic light scattering (DLS) electron microscope. Also, the encapsulation percentage and the release pattern of the extract were evaluated using a dialysis bag. Finally, its anticancer effects against breast cancer cell line (MCF_7) were studied. The results showed that the synthesized nanocarrier containing the extract has a spherical structure and its size is 104.1 nm and the encapsulation percentage is 62.35%, and the nanocarrier containing the extract has significant anticancer effects compared to the free extract. Conclusion: Nanomedicines such as Niosome are drug delivery systems that have been created to improve anticancer effects and especially to overcome the toxicity of standard systemic chemotherapy and are used as a targeted drug delivery system for anticancer purposes and it is suggested to use it with Other anticancer drugs and other cell lines were investigated in in vivo conditions.