Author/Authors
Çetintaş, V B Ege Üniversitesi - Tıp Fakültesi - Tıbbi Biyoloji Anabilim Dalı, Turkey , Oltulu, F Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , Kosova, B Ege Üniversitesi - Tıp Fakültesi - Tıbbi Biyoloji Anabilim Dalı, Turkey , Yavaşoğlu, A Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey , Oytun, E Ege Üniversitesi - Tıp Fakültesi - Fizyoloji Anabilim Dalı, Turkey , Aktuğ, H Ege Üniversitesi - Tıp Fakültesi - Histoloji ve Embriyoloji Anabilim Dalı, Turkey
Title Of Article
Effects of endothelin type-A receptor antagonist therapy on gene expressions of cell junctions and cell adhesion molecules in diabetic rat aorta
شماره ركورد
14792
Abstract
Aim: The aim of this study was to investigate the effects of endothelin receptor type-A antagonist (ERA-A), that has been applied for therapeutic reasons, on the gene expressions of cell junctions and cell adhesion molecules in the aorta of streptozotocin (STZ) induced diabetic rats.Materials and Methods: A total of 80 rats were analyzed in 4 equally distributed groups: Group 1: Control, Group 2: ERA-A administered, Group 3: Diabetic, and Group 4: Diabetic ERA-A treated. Diabetes was induced in 40 rats by a single injection of STZ and the animals were followed for 2 months. Twenty diabetic and 20 healthy rats were also treated with ERA-A at days 7 and 15. The expressions of genes coding for cell junctions and cell adhesion molecules in rat aortic tissue samples were analyzed by quantitative real-time reverse transcription-polymerase chain reaction.Results: The expressions of connexin-30 (Cx30), Cx33, Cx37, Cx40, and Cx45 were decreased in the diabetic group. A statistically significant increase in the expressions of Cx33 and Cx36 was determined in diabetic rats treated with ERA-A. When the diabetic group was compared to the control group a significant increase in the expression of α-1-catenin, and a significant decrease in the expression of β-1-catenin was found.Conclusion: In our study, diabetes induced damages in the intercellular communication of endothelial cells provided by gap junctions. Since administration of ERA-A has led to an increase in the expression of some connexin subtypes, its routine use in the treatment of this condition could be considered in the future.
From Page
88
NaturalLanguageKeyword
Diabetes , intercellular junctions , connexin , cell adhesion molecule , catenin , aorta
JournalTitle
Ege Journal Of Medicine
To Page
92
JournalTitle
Ege Journal Of Medicine
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