Author/Authors
DURAK, Zahide Esra Gazi Üniversitesi - Mühendislik Fakültesi - Kimya Mühendisliği Bölümü, TÜRKEY , GÜRÜ, Metin Gazi Üniversitesi - Mühendislik Fakültesi - Kimya Mühendisliği Bölümü, TÜRKEY
Title Of Article
ISOLATION, INHIBITION AND KINETIC MODELLING OF ALKALINE PHOSPHATASE (ALP) ENZYME
شماره ركورد
16642
Abstract
This study scopes the purification of alkaline phosphatase enzyme extracted from the liver and the investigation of kinetic properties for the reactions with and without inhibitor. Michaelis Menten and Lineweaver Burk graphs were drawn by means of experimental results. Km and Vmax values were calculated from these graphics. According to these values, substrate affinity (Km) and maximum velocity (Vmax) values were determined. Values without inhibitor; Km= 0,047 Vmax=24,87; with urea inhibitor Km= 0,054 Vmax=20,83 ; with creatinin inhibitor Km= 0,037 Vmax=20,83. The experiments were repeated with inhibitory substance and inhibition type was established. How high blood urea and creatinine levels affected alkaline phosphatase activity were determined. In this study, urea was found to cause noncompetetive inhibition and creatinine to cause uncompetetive inhibition on liver ALP.
From Page
209
NaturalLanguageKeyword
Alkaline phosphatase , Michaelis Menten , Lineweaver Burk , noncompetetive inhibition , uncompetetive inhibition.
JournalTitle
Journal Of The Faculty Of Engineering and Architecture Of Gazi University
To Page
215
JournalTitle
Journal Of The Faculty Of Engineering and Architecture Of Gazi University
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