Author/Authors
BULUT, Yasemin Fırat Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, TÜRKİYE , ANDİÇ, Şafak Fırat Üniversitesi - Tıp Fakültesi - Tıbbi Mikrobiyoloji Anabilim Dalı, TÜRKİYE
Title Of Article
Cloning of ORF2b Gene of TT Virus
شماره ركورد
17834
Abstract
Objective: Transfusion-transmitted virus (TTV) was first identified in patients with non-A to -G hepatitis following blood transfusion in 1997. The objective of this study is cloning of ORF2b gene fragments of TTV. Materials and Methods: In this study, the ORF2b genes from TTV DNA positive samples were amplified by polymerase chain reaction (PCR) and cloned into pGEM®-T plasmid vector. Results: The recombinant plasmid vectors including ORF2b gene were detected by PCRscreening test and restriction enzyme analysis. Conclusion: The producing of the gene clones containing ORF2b gene of TTV will contribute the enrichment of the viral gene library of our country. Moreover, the target proteins produced from the recombinant clones in future studies will be used in terms of the development of ELISA kits
From Page
63
NaturalLanguageKeyword
Transfusion , transmitted virus , TTV , cloning , PCR
JournalTitle
Fırat University Medical Journal Of Health Sciences
To Page
66
JournalTitle
Fırat University Medical Journal Of Health Sciences
Link To Document