PAMUK, Şebnem Afyon Kocatepşe University - Faculty of Veterinary Medicine - Department of Food Hygiene and Technology, TURKEY , GÜRLER, Zeki Afyon Kocatepşe University - Faculty of Veterinary Medicine - Department of Food Hygiene and Technology, TURKEY

Detection of prevalence and contamination level of Brucella spp. in local cheese produced in Afyonkarahisar, Turkey

18296

In this study, it was aim to determine presence and contamination level Brucella spp. in 100 home made fresh white cheese (not brined and aged cheese) with 50 home made and 50 dairy product totaly 100 tulum cheese (aged skin bag cheese) which were produced locally in Afyonkarahisar and sold at the bazaar. During this study, 200 cheese samples were evaluated in December 2011- January-February, 2012. Brucella spp. from cheese samples were isolated and identified according to Farell (1974), to determine the contamination level of Brucella MPN technique was performed. Brucella spp. were isolated all sample 14.2%. from all of the cheese samples (200). Contamination level of Brucella spp. in most samples was detected as 3.6 MPN/g, in five samples 3.6 MPN/g, in three samples 6.1 MPN/g, in four samples 7.4 MPN/g and in two samples 3.0 MPN/g. Brucella spp. were detected in 6 samples (6%) of tulum cheese and 8 samples (8%) of fresh white cheese. From all of the samples totally 28 isolates were obtained and 18 (64.2%) of them were Brucella abortus, 10 (35.7%) of them were Brucella melitensis. All of the isolated strains showed resistance to amoxicilline/clavulanic acid, penicilline, tetracycline, colistin and danofloxacin.

1

Antibiotic Resistance , Brucella abortus , Brucella melitensis , Brucella spp , Cheese , Raw Milk

Kocatepe Veterinary Journal

10