Author/Authors
Gürbüz, Burçak Marmara Üniversitesi - Eczacilik Fakültesi - Farmasötik Mikrobiyoloji Anabilim Dali, Turkey , Gürer, Ümran Soyoğul Marmara Üniversitesi - Eczacilik Fakültesi - Farmasötik Mikrobiyoloji Anabilim Dali, Turkey , Çevik, Özge Cumhuriyet Üniversitesi - Eczacilik Fakültesi - Biyokimya Anabilim Dali, Turkey , Yalçınkaya, İrfan Sağlık Bakanlığı - Süreyyapasa Gögüs Hastaliklari ve Gögüs Cerrahisi Egitim ve Arastirma Hastanesi, Turkey , Bekiroğlu, Gülnaz Nural Marmara Üniversitesi - Tip Fakültesi - Biyoistatistik veTibbi Bilisim Anabilim Dali, Turkey , Çevikbaş, Adile Marmara Üniversitesi - Eczacilik Fakültesi - Farmasötik Mikrobiyoloji Anabilim Dali, Turkey
Title Of Article
In vitro investigation of the effects of colistin alone with and tigecycline, imipenem and rifampicin combinations on polymorphonuclear leukocyte functions, oxidative stress, oxidant and antioxidant enzymes in patients with bronchiectasis.
شماره ركورد
18795
Abstract
In our study was investigated the in vitro effects of Colistin(4 μg/ml), alone with the combination of tigecycline(0.87 μg/ml), imipenem (30 μg/ml) and rifampicin (7 μg/ml) at therapeutic concentrations and malondialdehyde (MDA) level, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and myeloperoxidase (MPO) activity on polymorphonuclear leukocyte (PMN) functions (phagocytic and intracellular killing activity) of patients with bronchiectasis. PMNLs were isolated from venous blood by Ficoll-hypaque gradient centrifugation method. Phagocytic activity and intracellular killing activity were assayed by modified Alexander’s method. MDA levels were determinated by Beuge’s method, SOD, GSH-Px and MPO activities were assayed by Sun’s, Paglia and Valentine’s and Hillegas’s, methods respectively. All results are statistically evaluated. Phagocytic (p 0.0001), and intracellular killing activity (p=0.0113, p=0.0008, p=0.0014, p=0.0036)in the PMN of patients with bronchiectasis significantly increased when compared to the control. Enzyme activity (SOD, GSH-Px and MPO) and MDA level significantly decreased (p 0.001) in patients with bronchiectasis when compared to the control.
From Page
26
NaturalLanguageKeyword
Antioxidants , bronchiectasis , phagocytosis , oxidative stres
JournalTitle
Journal of Research in Pharmacy
To Page
35
JournalTitle
Journal of Research in Pharmacy
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