Author/Authors :
KAHRAMAN, Hüseyin Inonu University - Faculty of Art and Science - Department of Biology, Turkey , AYTAN, Emel Inonu University - Faculty of Art and Science - Department of Biology, Turkey , GİRAY KURT, Aslı Inonu University - Faculty of Art and Science - Department of Biology, Turkey , ÖZCAN, Duygu Inonu University - Faculty of Art and Science - Department of Biology, Turkey
Title Of Article :
Phenazine Production in The Presence of Heavy Metals in Recombinant Erwinia herbicola Bearing the Hemoglobin Gene
شماره ركورد :
25322
Abstract :
In this study, from Vitreoscilla sp. recombinant strains were obtained from cloned bacterial hemoglobin (VHb) gene (vgb) Eh [pUC8:15] and its wild-type strain Erwinia herbicola phenazine production in the presence of heavy metals were investigated. Time-dependent production of phenazine in the wild bacteria not shows a significant change. In generally, at the end of 96 hours in the presence of Pb in terms of production of wild-type bacteria phenazine 2.91 μg/ml of recombinant bacteria is to produce fenazin production 9.5 μg /ml, respectively. The average phenazine highest values were 7, 8.8, 8 and 9.5 μg/ml Cd, Co, Fe and Pb for Eh [pUC8:15]. E. herbicola is 6.32, 3.33, 6.02 and 2.99 μg/ml Cd, Co, Fe and Pb, respectively. Genetic engineering vgb, may be an effective method to increase phenazine production.
From Page :
11
NaturalLanguageKeyword :
Seconder metabolism , Erwinia herbicola , phenazine pigment , Vitreoscilla hemoglobin
JournalTitle :
Journal Of Natural an‎d Applied Sciences
To Page :
16
Link To Document :
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