Author/Authors :
Özkök, Elif Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Sinirbilim Anabilim Dali, Turkey , Kizmaz, Ahmet Nabi Sariyer Devlet Hastanesi, Turkey , Çakmakoglu, Bedia Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Moleküler Tip Anabilim Dali, Turkey , Aydogan, Hülya Yilmaz Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Moleküler Tip Anabilim Dali, Turkey , Eraltan, Ilhan Yaylim Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Moleküler Tip Anabilim Dali, Turkey , Öztürk, Oguz Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Moleküler Tip Anabilim Dali, Turkey , Aydin, Makbule Istanbul Üniversitesi - Deneysel Tip Arastirma Enstitüsü - Sinirbilim Anabilim Dali, Turkey
Abstract :
Objective: The purpose of our study was to examine the influence of piroxicam, non-steroid anti- inflammatory drug, on healing of ileal anastomoses in rats. Materials and Methods: 20 rats were divided randomly into two groups and submitted to resection and ileal anastomosis. The test group received piroxicam, 2mg/kg/day, beginning one day before operation until sacrification. The animals were sacrificed on third and seventh days after the operation. Tissue homogenate, indicator of tissue collagen content, was hydrolyzed with HQ and then evaporated to dryness. Evaporated sample was deproteinized in HC1 and after this processes hydroxyprolin was measured spectrophotometrically in supernatant fraction in which taken isopropanol. Results: In test group, hydroxyproline concentrations were slightly reduced on 3rd day but again increased to the preoperational levels on 7th day after anastomosis (p 0.05). On the other hand, in the control group, ileal hydroxyproline concentrations were reduced significantly on 3rd day but increased to preoperational levels on 7th day after the operation. Conclusion: In conclusion, piroxicam might improve hydroxyproline levels during initial phase of wound healing.
