Author/Authors :
CACINA, CANAN Istanbul Üniversitesi - DETAE Moleküler Tip A B D, Turkey , ARIKAN, SOYKAN Istanbul Egitim ve Arastirma Hastanesi - Genel Cerrahi Klinigi, Turkey , YILDIZ, YEMLIHA Istanbul Üniversitesi - DETAE Moleküler Tip A B D, Turkey , TOPTAS, BAHAR Istanbul Üniversitesi - DETAE Moleküler Tip A B D, Turkey , TURAN, SAIME Istanbul Üniversitesi - DETAE Moleküler Tip A B D, Turkey , ÖZKAN, N. EZGI Istanbul Üniversitesi - DETAE Moleküler Tip A B D, Turkey , KORKMAZ, GURBET Istanbul Üniversitesi - DETAE Moleküler Tip A B D, Turkey , KÜÇÜCÜK, SEDEN Istanbul Üniversitesi - Tip Fakültesi, Onkoloji Enstitüsü - Radyasyon Onkolojisi A B D, Turkey , YAYLIM, ILHAN Istanbul Üniversitesi - DETAE Moleküler Tip A B D, Turkey , ISBIR, TURGAY Yeditepe Üniversitesi - Tip Fakültesi - Tibbi Biyoloji A B D, Turkey
Abstract :
The role of Nitric Oxide (NO) in tumour biology is not well understood. However, it has been proposed that NO is a highly reactive free radical and plays important roles in growth, progression or metastasis of tumour. Nitric oxide synthases (NOSs) are a family of enzymes that catalyzing the production of (NO) by converting L-arginine to L-citrulline. The aim of present study was to examine Nitric Oxide Synthase 3 (NOS3) Glu298Asp polymorphism in patients with breast cancer. For this purpose, 49 patients with breast cancer and 89 healthy controls were included in the study. The NOS3 Glu298Asp polymorphism were genotyped by PCR-RFLP using peripheral blood samples in our study groups. NOS3 Glu298Asp genotype distribution in the breast cancer patients [GG: 2 (4.1%), GT: 22(44.9%), TT: 25(51%)] was statistically different from the healthy control group [GG: 25(28.1%), GT: 34(38.2%), TT: 30(33.7%)] (p=0.024). NOS3 Glu298Asp gene variation T allele frequency was significantly higher in breast cancer patients that of controls (χ2=7.229; p=0.007; OR: 1.228;%95 CI: 1.065-1.415). Conclusion: Our finding suggest that NOS3 Glu298Asp might be associated with breast cancer risk.
