Kavak, Dilek Demirbüker Afyon Kocatepe University - Engineering Faculty - Food Engineering Department, Turkey , Akdeniz, Bilge Afyon Kocatepe University - Engineering Faculty - Food Engineering Department, Turkey

Potential Application of Casein for the Inhibition of β‐Glucuronidase Activity

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β‐glucuronidase enzyme (EC. 3.2.1.31) is a hydrolytic enzyme and has an important role inglucuronidation mechanism. Changes in this enzyme concentration can lead development of adverse metabolic changes in the human body leading certain diseases and therefore inhibition researches for this enzyme are in the scope of different biomedical and nutritional applications. The aim of this study is to evaluate the inhibitory potential of casein towards β‐glucuronidase (GUS) enzyme and characterization of the inhibition of GUS enzyme by casein compound. Inhibition kinetics was carried out in a dose dependent way under simulated physiological conditions. GUS enzyme inhibition assay and measurements of kinetic parameters were used to analyze the inhibitory effect. Results showed that casein had high antioxidant activity and its activity depended on its concentration under simulated physiological conditions. Kinetic studies indicated that casein had a potential to be used as glucuronidase inhibitor and showed a mixed type inhibition mechanism towards the GUS enzyme.

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Inhibition , kinetic , casein , β‐glucuronidase

Afyon Kocatepe University Journal Of Science an‎d Engineering

78