مرکز منطقه ای اطلاع رساني علوم و فناوري - Using a desired gene activation or silencing methodology with gene transferring to stem cell in regenerative medicine

Abstract :

Human stem cell is a kind of special cell type which has self renewal capasity and differentiation potential. Embriyonic stem cells are an invaluable tool for stem cell biology and regenerative medicine as they have the capacity to differentiate into various functional cells. With the new gene technologies improved in recent years, it is possible to transfer a gene into a eukaryote cell. A desired gene can be activated or silenced via these technologies. In an appropriate condition, a differentiated human cell can be transformed into induced pluripotent stem cell by transforming the active form of some genes into the cell nucleus which are normally in inactivated form. As known, small interference RNA and micro RNA’s regulate the gene function in the cell. These molecules are generally transfected to eukaryote cells for gene silencing by using viral and non-viral transfection methodologies. Gene silencing technology is an important tool to drive induced pluripotent stem cell into a desired pathway becoming a differentiated neuronal cell, a pancreatic cell or a cardiac cell. These improvements may represent us a possibility of regeneration therapy applications in the future. This technology may provide us tissue and organ requirement in personal based conditions.