Yıldırım, Hülya Tosun İzmir Dr. Behçet Uz Çocuk Hastanesi - Patoloji Laboratuvarı, Turkey , Diniz, Gülden İzmir Dr. Behçet Uz Çocuk Hastanesi - Patoloji Laboratuvarı, Turkey , Aktaş, Safiye Dokuz Eylül Üniversitesi - Onkoloji Enstitüsü, Turkey , Olgun, Nur Dokuz Eylül Üniversitesi - Onkoloji Enstitüsü, Turkey

Investigation of the effects of Acetyl L-carnitine and doxorubicin on rats’ striated muscle

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Aim. Doxorubicin is a widely used anticancer agent which can cause an unpredictable cardiac toxicity increases with its cumulative dose. Previous studies demonstrated that side effects of doxorubicin can be reduced by L-carnitine. However in only a few studies, toxic effects of doxorubicin on skeletal muscles was investigated widely. The objective of present study was to investigate the effects of doxorubicin and Acetyl L-carnitine on skeletal muscles in rats. Method. The study was performed on adult male Wistar-Albino rats which are 200-300 gr in weight. Rats were divided to four groups as control, doxorubicine, ALCAR and ALCAR + doxorubicine. The first group (n=7) was the control. The second and forth groups (n=6/n=6) received intraperitoneal doxorubicine (cumulative dose 15 mg/kg). Third and forth groups (n=7/n=6) received intraperitoneal ALCAR (300 mg/kg/day) and it was continued 10 days. All animals in each group were sacrificed on tenth day of the study and muscle biopsy samples were taken from quadriceps muscles. All samples were frozen and 8-micron sections were cut using cryostat. Slides were stained with routine hematoxylin eosin, enzyme histochemically combined COX and SDH, histochemically with modified Gomori’s trichrome, PAS and oil red O. Fast myosin antibody was used for discriminating myofiber type. Results. No significant differences were found in muscle fiber type distribution, lipid and glucogen accumulation, or mitocondrial function in Doxorubicine or ALCAR groups compared with control group. Conclusion. This study indicated that treatment with doxorubicine or ALCAR cannot produce significant injury to non-cardiac striated muscle tissue.

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Doxorubicin , ALCAR , skeletal muscle , rat

Cumhuriyet Medical Journal

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