Author/Authors :
Saleh, Manal National Commission for Biotechnology (NCBT) - Medical Biotechnology Laboratory, Syria , Hajjar, Jamal University of Damascus - Faculty of Agriculture - Department of Plant Protection, Syria , Rahmo, Abdulkader National Commission for Biotechnology (NCBT) - Medical Biotechnology Laboratory, Syria
Title Of Article :
EFFECT OF SELECTED INSECTICIDES ON SF9 INSECT CELL LINE
Abstract :
The toxic effect of three insecticides: dimethoate (organophosphate insecticide), acetamiprid (neonicotinoid insecticide) and deltamethrin (pyrethroid insecticide) were evaluated in vitro on cultured Sj9 cell line. Cell growth inhibition was measured by the 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Regression Analysis was used to estimate the 20% inhibition of cells growth (IC20). The IC20 values obtained for deltamethrin, acetamiprid and dimethoate were: 46.8, 61.6 and 68.9 μM, respectively. The proportion of phagocytic cells was positively correlated with the applied concentrations of the insecticides.
NaturalLanguageKeyword :
Sf9 insect cell line , insecticide , MTT assay , cell toxicity
JournalTitle :
Lebanese Science Journal
