Title :
Bioreduction of chloroaurate ions using fruit extract Punica granatum (Pomegranate) for synthesis of highly stable gold nanoparticles and assessment of its antibacterial activity
Author :
Basavegowda, Nagaraj ; Sobczak-Kupiec, Agnieszka ; Fenn, Rebecca ; Dinakar, Salman
Author_Institution :
Dept. of Biotechnol., Shridevi Inst. of Eng. & Technol., Tumkur, India
Abstract :
The design, synthesis and characterisation of biologically synthesised nanomaterials have become an area of significant interest. Presented is a report on the extracellular synthesis of gold nanoparticles using Punica granatum (Pomegranate) fruit extract as the reducing agent to synthesise Au nanoparticles. On treating aqueous chloroauric acid solutions with P. granatum fruit extract, rapid reduction of the chloroaurate ions is observed, leading to the formation of highly stable gold nanoparticles in solution. These nanoparticles showed an absorption peak at 536 nm in the UV-vis spectrum corresponding to the plasmon resonance of gold nanoparticles. Scanning electron microscopy analysis of the gold nanoparticles indicated that they ranged in size from 10 to 50 nm. The Fourier transform infrared spectroscopy spectrum confirmed the presence of main groups occurred in natural plant extract from P. granatum. The synthesised gold nanoparticles were active against Streptobacillus sp. and Escherichia coli.
Keywords :
Fourier transform spectra; antibacterial activity; biochemistry; cellular biophysics; gold; infrared spectra; microorganisms; nanofabrication; nanomedicine; nanoparticles; reduction (chemical); scanning electron microscopy; surface plasmon resonance; ultraviolet spectra; visible spectra; Au; Escherichia coli; Fourier transform infrared spectroscopy; P. granatum fruit extract; Pomegranate; Punica granatum fruit extract; Streptobacillus sp; UV-visible spectrum; antibacterial activity; aqueous chloroauric acid solutions; biologically synthesised nanomaterials; bioreduction; chloroaurate ions; extracellular synthesis; gold nanoparticles; natural plant extract; plasmon resonance; reducing agent; scanning electron microscopy;
Journal_Title :
Micro & Nano Letters, IET
DOI :
10.1049/mnl.2013.0137