Title :
Scanning confocal near-infra-red microscopy: a new microscopy technique for three-dimensional histopathology
Author :
Murthy, M.S.N. ; Jones, M.G. ; Kulka, J. ; Davies, J.D. ; Halliwell, M. ; Jackson, P.C. ; Bull, D.R. ; Wells, P.N.T.
Author_Institution :
Dept. of Clinical Radiol., Bristol Univ., UK
fDate :
10/1/1995 12:00:00 AM
Abstract :
To image microstructures within tissue in detail using conventional light microscopy it is necessary to process the tissue and obtain thin (≈5 μm) physical sections by using destructive physical sectioning techniques. This article describes a microscopy technique, using near-infra-red light (700-1500 nm) as the source, which is able to image thin sections of thick-tissue specimens nondestructively. Images of contiguous two-dimensional thin sections obtained serially can be combined to generate a three-dimensional image of the structures being imaged
Keywords :
biological techniques; biomedical imaging; infrared imaging; optical microscopy; 5 mum; 700 to 1500 nm; contiguous two-dimensional thin sections; microstructures imaging; nondestructive imaging; scanning confocal near-infra-red microscopy; thick-tissue specimens; three-dimensional histopathology; three-dimensional image;
Journal_Title :
Engineering Science and Education Journal
DOI :
10.1049/esej:19950509
