Handling of a single DNA molecule using electric field and laser beam

Author

Mizuno, Akira ; Nishioka, Masateru ; Tanizoe, Tomoki ; Katsura, Shinji

Author_Institution

Dept. of Ecological Eng., Toyohashi Univ. of Technol., Japan

Volume

31

Issue

6

fYear

1995

Firstpage

1452

Lastpage

1457

Abstract

An accurate handling of a single DNA molecule will support a novel technique for sequencing of large DNA. The opto-electrostatic micromanipulation (OEMM) has been applied to handle a single DNA molecule. The DNA molecule frequently changes its position and its shape due to Brownian motion, therefore precise handling becomes difficult. Gelation of the sample by agarose gel is effective to suppress Brownian motion. Fundamental handling techniques for a single DNA molecule in agarose gel were demonstrated as follows: (1) fixation with stretched shape, (2) cutting by ultraviolet laser, and (3) isolation and recovery into the capillary (inner diameter 18 μm). Amplification of the recovered DNA fragment by the PCR (polymerase chain reaction) method is the next step of this study. This micromanipulation technique may contribute for accelerating the time needed for DNA sequencing

Keywords

DNA; bioelectric phenomena; biological techniques; biotechnology; electric fields; electrophoresis; electrostatics; molecular biophysics; radiation pressure; Brownian motion suppression; DNA molecule handling; agarose gel; capillary; electric field; laser beam; opto-electrostatic micromanipulation; polymerase chain reaction method; recovered DNA fragment amplification; sample gelation; stretched shape fixation; ultraviolet laser cutting; Biomedical optical imaging; DNA; Dielectric measurements; Dielectrophoresis; Electrokinetics; Electrostatics; Industry Applications Society; Microscopy; Optical feedback; Shape;

fLanguage

English

Journal_Title

Industry Applications, IEEE Transactions on

Publisher

ieee

ISSN

0093-9994

Type

jour

DOI

10.1109/28.475740

Filename

475740