Development of a new geometrical form of micropipette: electrical characteristics and an application as a potassium ion selective electrode

Using a mix of thermal and anodic bonding together with microlithographic techniques, the safe transference of a Si ₃N ₄ film with a pore (diameter down to 1 mu m) to a glass tube tip (external diameter 800 mu m) was accomplished, yielding a new geometrical form of micropipette. Compared with conventional glass micropipettes the device has shown lower resistance, more stable capacitance (independent of the tip immersion depth), tip potential closer to that of a salt bridge, and a simplified filling process. Using this device as a potassium ion selective electrode (ISE), a faster response time ISE was achieved. These features indicate that the new device can advantageously substitute the conventional glass micropipettes when cell impalement is not required.