DocumentCode :
1321420
Title :
Effects of poly(lactic-co-glycolic acid) on preparation and characteristics of plasmid DNA-loaded solid lipid nanoparticles
Author :
Zhu, L. ; Xie, Shengli ; Dong, Zhaoyang ; Wang, Xiongfei ; Wang, Yannan ; Zhou, Weicheng
Author_Institution :
Dept. of Preventive Veterinary Med., China Agric. Univ., Beijing, China
Volume :
5
Issue :
3
fYear :
2011
fDate :
9/1/2011 12:00:00 AM
Firstpage :
79
Lastpage :
85
Abstract :
Poly(lactic-co-glycolic acid) (PLGA) was used as a polymeric emulsifier to encapsulate plasmid DNA into hydrogenated castor oil (HCO)-solid lipid nanoparticles (SLN) by w/o/w double emulsion and solvent evaporation techniques. The effects of PLGA on the preparation, characteristics and transfection efficiency of DNA-loaded SLN were studied. The results showed that PLGA was essential to form the primary w/o emulsion and the stability of the emulsion was enhanced with the increase of PLGA content. DNA-loaded SLN were spherical with smooth surfaces. The SLN had a negative charge in weak acid and alkaline environment but acquired a positive charge in acidic pH and the cationisation capacity of the SLN increased with the increase of PLGA/HCO ratio. Agarose gel electrophoresis demonstrated that the majority of the DNA maintained its structural integrity after preparation and being extracted or released from DNA-loaded SLN. When PLGA/HCO ratio increased from 5 to 15%, the encapsulation efficiency, loading capacity and transfection efficiency of the nanoparticles increased significantly, whereas the changes of particle size and polydispersity index were insignificant. Cytotoxicity study in cell culture demonstrated that the SLN was not toxic.
Keywords :
DNA; biological techniques; cellular biophysics; electrophoresis; molecular biophysics; nanoparticles; pH; particle size; polymers; Agarose gel electrophoresis; DNA-loaded SLN; HCO-solid lipid nanoparticles; PLGA content; acidic pH; alkaline environment; cationisation capacity; cell culture; cytotoxicity; double emulsion; encapsulation efficiency; hydrogenated castor oil; particle size; plasmid DNA-loaded solid lipid nanoparticles; poly(lactic-co-glycolic acid); polydispersity index; polymeric emulsifier; solvent evaporation techniques; transfection efficiency; weak acid;
fLanguage :
English
Journal_Title :
Nanobiotechnology, IET
Publisher :
iet
ISSN :
1751-8741
Type :
jour
DOI :
10.1049/iet-nbt.2010.0041
Filename :
6019065
Link To Document :
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