Multi-Channel SQUID-Based Ultra-High-Sensitivity In-Vitro Detections for Bio-Markers of Alzheimer´s Disease Via Immunomagnetic Reduction

Author

Chiu, M.J. ; Horng, H.E. ; Chieh, J.J. ; Liao, S.H. ; Chen, C.H. ; Shih, B.Y. ; Yang, C.C. ; Lee, C.L. ; Chen, T.F. ; Yang, S.Y. ; Hong, C.Y. ; Yang, H.C.

Author_Institution

Dept. of Neurology, Nat. Taiwan Univ., Taipei, Taiwan

Volume

21

Issue

3

fYear

2011

fDate

6/1/2011 12:00:00 AM

Firstpage

477

Lastpage

480

Abstract

Via immunomagnetic reduction assay, bio-molecules can be quantitatively detected with the aid of bio-functionalized magnetic nanoparticles, which are used as labeling markers for specific bio-molecules. To achieve an ultra-high sensitivity in the detection of bio-molecules, the superconducting quantum interference device (SQUID) looks very promising as a sensor for the magnetic signal that are related to the concentration of the detected bio-molecules. We had developed a single channel SQUID-based magnetosusceptometer, but for increasing the detection through-put, we have developed a multi-channel SQUID-based magnetosusceptometer. In this work, the design and the working principle of a 4-channel SQUID-based magnetosusceptometer are introduced. By utilizing scanning technology, 4 samples can be logged into a SQUID-based magnetosusceptometer simultaneously. Be noted that only one single SQUID magnetometer has been used in the magnetosusceptometer. The precision and the sensitivity of detecting bio-molecules by using a 4-channel SQUID-based magnetosusceptometer have been investigated. The detected bio-molecules are biomarkers for Alzheimer´s disease.

Keywords

SQUID magnetometers; biomedical equipment; biomedical materials; biosensors; diseases; magnetic particles; magnetic susceptibility; molecular biophysics; nanobiotechnology; nanoparticles; neurophysiology; patient diagnosis; Alzheimer´s disease biomarkers; biofunctionalized magnetic nanoparticles; biomolecule concentration; biomolecules; immunomagnetic reduction assay; magnetic signal biosensor; multichannel SQUID based magnetosusceptometer; scanning technology; superconducting quantum interference device; ultrahigh sensitivity in vitro biomarker detections; Coils; Immune system; Magnetic resonance; Magnetic resonance imaging; Magnetometers; Nanoparticles; Superconducting magnets; Alzheimer´s disease; SQUIDs; immunoassay; magnetic nanoparticles;

fLanguage

English

Journal_Title

Applied Superconductivity, IEEE Transactions on

Publisher

ieee

ISSN

1051-8223

Type

jour

DOI

10.1109/TASC.2010.2087304

Filename

5634078