Development and calibration of a microfluidic biofilm growth cell with flow-templating and multi-modal characterization

Author

Paquet-Mercier, Francois ; Karas, Adnane ; Safdar, Muhammad ; Aznaveh, Nahid Babaei ; Zarabadi, Mirpouyan ; Greener, Jesse

Author_Institution

Dept. de Chim., Univ. Laval, Quebec City, QC, Canada

fYear

2014

fDate

26-30 Aug. 2014

Firstpage

1557

Lastpage

1562

Abstract

We report the development of a microfluidic flow-templating platform with multi-modal characterization for studies of biofilms and their precursor materials. A key feature is a special three inlet flow-template compartment, which confines and controls the location of biofilm growth against a template wall. Characterization compartments include Raman imaging to study the localization of the nutrient solutions, optical microscopy to quantify biofilm biomass and localization, and cyclic voltammetry for flow velocity measurements. Each compartment is tested and then utilized to make preliminary measurements.

Keywords

bioMEMS; biomedical materials; flow measurement; microfluidics; optical microscopy; velocity measurement; voltammetry (chemical analysis); Raman imaging; biofilm biomass; biofilm localization; characterization compartments; cyclic voltammetry; flow velocity measurement; inlet flow-template compartment; microfluidic biofilm growth cell; microfluidic flow-templating platform; multimodal characterization; nutrient solutions; optical microscopy; template wall; Biomedical optical imaging; Electrodes; Microchannels; Microfluidics; Optical imaging; Optical variables measurement; Surface treatment;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE

Conference_Location

Chicago, IL

ISSN

1557-170X

Type

conf

DOI

10.1109/EMBC.2014.6943900

Filename

6943900